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Fig. 5 | SpringerPlus

Fig. 5

From: Simultaneous quantification of salivary 3-hydroxybutyrate, 3-hydroxyisobutyrate, 3-hydroxy-3-methylbutyrate, and 2-hydroxybutyrate as possible markers of amino acid and fatty acid catabolic pathways by LC–ESI–MS/MS

Fig. 5

Representative SRM chromatograms of 2PM-derivatized 3HB, 2HIB, 3HMB, and 2HB in 5 µL of saliva from a healthy human. a m/z 196 g m/z 110 for 2PM-3HB, 2PM-3HIB and 2PM-2HB. b m/z 210 g m/z 192 for 2PM-3HMB. c m/z 200 g m/z 110 for 2PM-[13C4]3HB. The peaks for 3HB, 3HIB, 2HB and 3HMB in chromatograms correspond to 650 fmol (6.5 µM), 350 fmol (3.5 µM), 450 fmol (4.5 µM) and 70 fmol (0.7 µM), respectively. The numbers above the SRM ion pair represent the full scale of the chromatogram. SRM selected reaction monitoring; and see legends of Figs. 1, 2, 3, 4 for other abbreviations

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