Fig. 4

GFP transcription in T. crassiceps microinjected cysticerci. a RT-PCR performed on total RNA from GFP-TOPO or water microinjected cysts and lipofectamine transfected HEK 293 cells; RNA was isolated 24 h post microinjection/transfection, then, a nested PCR amplification was made using two sets of GFP primers. Lanes: 1 DNA size markers; 2 HEK 293 cells transfected with GFP-TOPO plasmid; 3 and 4 T. crassiceps cysts microinjected with GFP-TOPO plasmid or water, respectively. b Plasmid contamination control for the total RNAs used in a. Lanes: 1 DNA size markers; 2 and 3 Nested PCR amplifications (excluding the reverse transcriptase reaction) using the same sets of GFP primers on total RNA from T. crassiceps cysts microinjected with GFP-TOPO plasmid, and from HEK 293 GFP-TOPO transfected cells, respectively