Somatic embryogenesis and plant regeneration of cassava (Manihot esculenta Crantz) landraces from Cameroon

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Table 4 Effect of cytokinins and photoperiod on callus and bud induction from green somatic embryos of five cassava cultivars from Cameroon

Var	Cyt	Frequency (%) of callus induction		Frequency (%) of bud induction		Average number of buds per explant
Var	Cyt	Photoperiod	Dark	Photoperiod	Dark	Photoperiod	Dark
SK	BAP	91.00 ± 3.71 a	90.00 ± 3.33 a	69.00 ± 6.74 a	56.00 ± 7.18 a	13.48 ± 0.43 abcd	07.97 ± 1.20 cdef
SK	TDZ	0.00 ± 0.00 b	0.00 ± .00 b	12.00 ± 3.26 de	16.00 ± 5.81 cde	02.40 ± 0.70 e	0.90 ± 0.3 1 f
NM	BAP	81.00 ± 6.04 a	92.00 ± 4.42 a	53.00 ± 5.17 ab	66.00 ± 4.26 a	08.30 ± 0.72 ce	06.90 ± 0.30 def
NM	TDZ	0.00 ± 0.00 b	0.00 ± 0.00 b	0.00 ± .00 e	06.40 ± 2.97 e	0.00 ± .00 f	0.80 ± 0.29 ef
EW	BAP	81.77 ± 6.30 a	86.00 ± 3.05 a	71.55 ± 9.59 a	54.30 ± 7.38 ab	11.30 ± 0.74 bcde	06.48 ± 0.86 def
EW	TDZ	0.00 ± 0.00 b	0.00 ± 0.00 b	20.00 ± 0.00 cde	28.00 ± 7.42 cd	5.00 ± 0.00 ef	0.90 ± 0.23 ef
RL	BAP	100.00 ± 0.00	100.00 ± 0.00	56.00 ± 6.53 a	54.00 ± 5.81 ab	18.82 ± 1.52 ab	06.30 ± 0.65 def
RL	TDZ	0.00 ± 0.00 b	06.00 ± 3.05 b	10.00 ± 3.33 de	34.00 ± 7.91 bc	0.50 ± 0.16 f	02.45 ± 0.72 ef
ER	BAP	100.00 ± 0.00	100.00 ± 0.00	62.00 ± 5.53 a	62.70 ± 5.20 a	19.67 ± 2.70 a	15.26 ± 2.22 abc
ER	TDZ	0.00 ± 0.00 b	0.00 ± 0.00 b	0.00 ± .00 e	06.40 ± 2.97 e	0.00 ± .00 f	0.80 ± 0.29 ef

Within the same column, mean values followed by the same letter are not significantly different at α = 5 % (Newman–Keuls test)
±, standard deviation; Var, varieties; Cyt, cytokinins