Fig. 1

Regeneration of cassava cultivars from Cameroon. Apical immature meristematic leaf lobe explants (A) induced compact non-embryogenic callus (B) and callus with proembryogenic masses (C). Clusters of organized embryogenic structures consisting of globular (D) heart and torpedo structures (E), early cotyledonary stage (F), asynchronous development of somatic embryos (G) and green cotyledon (H) were observed. Organogenic callus with buds (I) derived from green cotyledons developed clusters of shoot buds (J). Elongated shoot buds rooted and developed into whole plantlets (K) in vitro. After transferring in boxes, hardened plantlets (L) were acclimatized (M) and they established in the greenhouse and grew into normal plants (N)