Seasonal and inter-annual variation in the chlorophyll content of three co-existing Sphagnum species exceeds the effect of solar UV reduction in a subarctic peatland
© Hyyryläinen et al. 2015
Received: 23 April 2015
Accepted: 17 August 2015
Published: 4 September 2015
We measured chlorophyll (chl) concentration and chl a/b ratio in Sphagnum balticum, S. jensenii, and S. lindbergii, sampled after 7 and 8 years of ultraviolet-B (UVB) and temperature manipulation in an open field experiment in Finnish Lapland (68°N). We used plastic filters with different transmittance of UVB radiation to manipulate the environmental conditions. The plants were exposed to (1) attenuated UVB and increased temperature, (2) ambient UVB and increased temperature and (3) ambient conditions. Chlorophyll was extracted from the capitula of the mosses and the content and a/b ratio were measured spectrophotometrically. Seasonal variation of chlorophyll concentration in the mosses was species specific. Temperature increase to 0.5–1 °C and/or attenuation of solar UVB radiation to ca. one fifth of the ambient (on average 12 vs. 59 uW/cm2) had little effect on the chlorophyll concentration or its seasonal variation. In the dominant S. lindbergii, UVB attenuation under increased temperature led to a transient decrease in chlorophyll concentration. Altogether, species-specific patterns of seasonal chlorophyll variation in the studied Sphagna were more pronounced than temperature and UVB treatment effects.
KeywordsSphagnum balticum Sphagnum jensenii Sphagnum lindbergii Chlorophyll UVB Peatlands
Sphagna are crucial in forming and sustaining peatlands—specific ecosystems that maintain a unique diversity of habitats and species (Clymo and Hayward 1982; Vitt 2000; Rydin et al. 2006; Rydin and Jeglum 2006) and function as a large store of organic carbon, particularly in boreal and subarctic areas (Gorham 1991; Limpens et al. 2008). As a key component of the ecosystem, they affect many processes within it; therefore, the changes triggered in Sphagna by a changing environment may be reflected in the entire ecosystem. This is important, as they are also susceptible to changes in the environment, including shifts in UVB and temperature regime (e.g. Dorrepaal et al. 2003; Huttunen et al. 2005; Wiedermann et al. 2007; Gignac 2011), principally due to their specific structure (leaves of one cell layer, absence of waxes or any other protective surface structures). In open peatlands, Sphagnum mosses are not shielded by tree canopies and can be exposed to high levels of UVB irradiance. Given the importance of Sphagna for the sustainability of peatlands, considering recent increased solar UVB irradiance (Weatherhead et al. 2005) and elevated temperatures at high latitudes (Jylhä et al. 2004; McBean 2005), it is essential that we understand how both these factors affect peat mosses.
Solar UV radiation is an important environmental factor, regulating growth, biochemistry and some structural features in bryophytes (Gehrke 1998; Rozema et al. 2005; Caldwell et al. 2007). UV effects on mosses are species-specific, possibly reflecting differences in desiccation tolerance (Csintalan et al. 2001). Sphagnum mosses growing under near-ambient conditions seem to be able to hold more water as they are shorter and stouter, and have larger capitula, than those growing under attenuated UVB (Robson et al. 2003, 2004). Changes in the intensity of different wavelengths of solar irradiance affect shape and size of chloroplasts (Glime 2007), as well as pigment concentration in bryophytes (Niemi et al. 2002a, b).
Even a small enhancement (by less than 1 °C) of summer temperatures may have a significant effect on growth and cover density of peat mosses (Dorrepaal et al. 2003). These responses are species-specific, and seem to correlate with the requirements of a particular species to environmental conditions (Gunnarsson et al. 2004; Breeuwer et al. 2008; Haraguchi and Yamada 2011). Differences in responses to increased temperatures may, in the long run, lead to changes in species composition in a given ecosystem. Where increased temperatures do not directly affect peatmosses, they may still have an impact on vascular plants (Weltzin et al. 2000). As a consequence, species range and ratio may change, and that may be hazardous for the ecosystem.
The photosynthetic pigments in Sphagna are chlorophyll a and b, and carotenoids. The absorption spectra of the two chlorophylls (chls) differ slightly (Porra et al. 1989), and plants use these differences to adapt to varying light conditions. In mosses, the total chl content and a/b ratio changes depending on factors such as light availability, plant vitality, growth phase, and various environmental stresses (Baxter et al. 1992; Martínez-Abaigar et al. 1994; Gerdol 1996; Martínez-Abaigar and Núñiez-Olivera 1998; Bonnett et al. 2010). A deficit of light often promotes chl b production, reducing the chl a/b ratio. Total chl content is generally higher in species that grow in shady habitats, and lower in those commonly found in open sunny sites (Martin and Churchill 1982; Martínez-Abaigar and Núñiez-Olivera 1998). During the growing season, the chl content normally increases with decreasing light intensity. Seasonal pigment variation is more pronounced in species growing in varying light conditions, than those growing in permanently shady or sunny habitats (Kershaw and Webber 1986; Martínez-Abaigar and Núñiez-Olivera 1998). In Sphagna, chl content is species-specific (Gehrke 1998; Niemi et al. 2002a, b) and it depends on the ecological requirements of the species (Yefremov et al. 2000; Naumov and Kosykh 2011). Night chilling may cause rapid degradation of chls in some Sphagna (Gerdol et al. 1994).
In Sphagnum mosses, a positive correlation between chl concentration and net photosynthesis has been recorded (Gaberščik and Martinčič 1987). Thus, environmental factors affecting the chl content in peat mosses may also affect the rate of photosynthesis. Bryophytes from open sunny habitats are normally adapted to photosynthesise under intensive sunlight, some reaching P max at ~1000 µmol m−2 s−1 and dissipating extensive energy by non-photochemical quenching, NPQ (Proctor 2000). However, those species in open environments often experience water deficit. On the other hand, bryophytes from shady forest sites are adapted to live under as little as ~100–300 µmol m−2 s−1 but more rarely have to sustain drought. In this sense, Sphagna species typical for lawns and carpets of open bogs have a very specific position, as they grow under intensive light usually yet with no water deficit. However, Sphagnum species of open peatlands are reported to have lower photosynthetic capacity and maximum quantum yield than those that grow in the shade (Hájek et al. 2009).
Synergetic effects of solar UV with other co-varying environmental factors in bryophytes may be more pronounced (Sonesson et al. 2002; Rinnan et al. 2013) or even opposite (Björn et al. 1999) to those of UV alone. However, studies of such combined effects are scarce. Little is known about responses of different Sphagnum species to simultaneous changes in solar UV and temperature regime over a prolonged period of time (Sonesson et al. 2002; Aphalo 2003). UVB attenuation may have similar effects on peatmosses as increased summer temperatures, that include e.g. increasing length increment and simultaneously decreasing the bulk density of moss cover (Dorrepaal et al. 2003; Robson et al. 2003, 2004). To study the impact of UVB and temperature on peatmosses, we conducted an open field experiment on a flark fen in northern Finland. We aimed to study how changes in both UVB and temperature regime affect concentrations of chl a and b, and their ratio in three Sphagnum species: S. balticum, S. jensenii and S. lindbergii. Although varying in coverage, these were the most common peat moss species on the experimental site.
We hypothesized that under attenuated solar UVB and slightly elevated temperature chlorophyll production in Sphagnum mosses would increase. We suggested that UVB-attenuation plots might provide the most favourable conditions for plant growth, because of the release of apparent negative UVB effects and a parallel increase in temperature. Additionally, the polyester filters used for creating −UVB conditions partly transmit UVA, which may have positive effect on plants (Niemi et al. 2002b). Hence, we expected a higher chlorophyll concentration in the mosses growing in the UVB-attenuation plots.
The UVB attenuation experiment took place in northern Finland on a remote oligotrophic tall-sedge Sphagnum flark fen, in Kaltiovuoma, Vuotso, about 200 km from Polar Circle [263 m a.s.l., 68˚10′N, 26˚42′E (75649:34878)] (Soppela et al. 2006). This open field experiment was a part of the ECOREIN project (The ecological and socioeconomical responses of global change on reindeer pastures). Therefore, the experimental site represented a typical reindeer summer pasture, where peat mosses dominated in the ground layer, most abundant being S. lindbergii, S. jensenii, S. angustifolium, and S. balticum. The field layer was characterized by sedges (Eriophorum russeolum, E. angustifolium, Carex rotundata, C. rostrata, C. limosa, C. magellanica subsp. irrigua, Scheuchzeria palustris), herbs (Menyanthes trifoliata, Rubus chamaemorus) and shrubs (Andromeda polifolia, Betula nana, Vaccinium oxycoccus, V. microcarpum). Chemical analysis of the upper substrate layer (0–30 cm) gave the following results: pH 4.2, carbon (total organic carbon) 41.5 %, nitrogen 0.64 %, carbon/nitrogen ratio 65, ammonium (NH4) 82.9 mg kg−1, nitrate (NO3) <0.2 mg kg−1 and ammonium-lactate extracted (i.e., plant-available) nutrients: phosphorous 45 mg kg−1, calcium 1.680 mg kg−1, magnesium 568 mg kg−1 and potassium 133 mg kg−1 (Soppela et al. 2006; Martz et al. 2009).
Each plot was equipped with a portable temperature datalogger (Tiny Talk II, −40 to 75 °C; OTLM Software, Orion Components, Ltd., Chichester, UK), attached to the central pole about 15 cm below the filter and 15 cm above the surface of the Sphagnum layer at the beginning of each growing season. Temperature was recorded every 1.5 h. Due to the film cover, the temperature in −UVB and control plots increased up to 1 °C, compared to the ambient plots. The average temperature for the period of record in 2008 (14th June–27th August) was 13.8 ± 0.09 °C in ambient, 14.8 ± 0.09 °C in control, and 14.5 ± 0.09 °C in –UVB plots (mean ± SE). Differences in temperature among the three treatments were statistically significant (F2,26 = 30.5, p < 0.001).
Sampling and measurements
For chlorophyll analysis, we chose S. lindbergii, S. jensenii, and S. balticum, which make up, respectively 87, 5 and 3 % of the ground cover in the experimental plots (cover percentages calculated as a mean value from the 30 sample plots). We sampled S. lindbergii (n = 8–10 samples per treatment) in 2007 (12 July, 26 July, 23 August, 27 September) and 2008 (13 June, 3 July, 24 July, 28 August) to follow both seasonal and year-to-year changes in chlorophyll concentration. Each sample is a pooled sample of 10 capitula collected from each of the 30 experimental plots. When analysing year-to-year changes in chl concentration, differences in the sampling dates between the 2 years had to be taken into account. To analyse interspecies and seasonal variation, we also collected S. balticum and S. jensenii (n = 5–10 per treatment, due to smaller coverage of the species in the plots) in 2008 (3 July, 24 July, 28 August). The samples were immediately immersed in liquid nitrogen and transported to the laboratory of the Natural Resources Institute Finland (Luke) in Rovaniemi. The material was kept in a super freezer (−80 °C) prior to analysis. It was then lyophilized and ground. Exactly 15 mg (DW) of each sample were scaled, and used for chlorophyll extraction with pure methanol (1.5 ml per sample) in 24 h, at +4 °C, in the dark. The absorbance of the methanol extract was measured at 652.0, 665.2 and 750 nm with a spectrophotometer (Shimadzu-1700), and the concentration of chlorophyll a and b, and total chlorophyll (µg ml−1) was calculated following Porra et al. (1989).
Seasonal and inter-annual variation
Effect of UVB treatment, species, and sampling date on concentration of chlorophyll a and b, total chlorophyll (a + b) and a/b ratio
Chl a + b
Treatment × species
Treatment × date
Species × date
Treatment × species × date
Effect of UVB treatment and sampling date on concentration of chlorophyll a and b, total chlorophyll (a + b) and a/b ratio in Sphagnum lindbergii
Chl a + b
Treatment × date
In 2008, concentration of chlorophyll in the dominant S. lindbergii increased sharply from very low concentrations in mid-June to between two and six times that amount by July, then remained fairly stable for the rest of the season. In both S. jensenii and S. balticum, chlorophyll concentration decreased significantly towards the end of the summer in all the treatments (Fig. 5). S. balticum had significantly lower chlorophyll values than the other two species.
In S. lindbergii, chlorophyll concentration was noticeably lower in 2007 compared to 2008 (Fig. 5c). It also varied significantly during the growing season of 2007, being highest in July, decreasing at the end of summer and increasing again in September. The chl a/b ratio in this species was significantly lower in 2007 than in 2008 (Fig. 6).
Throughout the experiment, combined temperature and UVB manipulation had only minor effects on the chosen Sphagnum species in this habitat, and these effects were date-dependent (Tables 1, 2; treatment effect includes both UVB and temperature manipulation). A transient UV effect was detected on the 3rd July 2008 in S. lindbergii as a higher chl a + b concentration in the plants under control conditions, compared to those in the UVB-attenuated treatment (Fig. 5c).
A significant temperature effect was observed in S. lindbergii in 2008 (Figs. 5c, 6). By the end of the growing season in 2007 (27th September), plants under different treatments had approximately the same concentration of total chlorophyll and chl a/b ratio. However, when the next season started (13th June 2008), chlorophyll concentration and a/b ratio increased significantly in only the ambient plots, while in the plants under the filters these parameters remained at the level of the previous year. By contrast, later in the season of 2008, the chlorophyll concentration increased sharply (four–six fold) in S. lindbergii under the filters, while in ambient conditions less so (twofold). The chl a/b ratio in this species remained stable in ambient conditions for the whole growing season in 2008, while in the plots with increased temperature the ratio was significantly lower at the beginning of the season (Fig. 6). Additionally, the temperature effect was observed in S. lindbergii on 23rd August 2007, as a higher chl a/b in ambient compared to control plots.
Correlation between pH of the substrate and chlorophyll content in the three co-existing Sphagnum species in ambient and experimentally altered temperature and UVB conditions
pH in a treatment
Temperature and light intensity together affect photosynthetic rates in mosses (Proctor 2011), and thus may have potential to affect chlorophyll content in Sphagna; temperature effects on photosynthetic pigments in peat mosses depend on light intensity (Haraguchi and Yamada 2011). Certain Sphagnum species are adapted to have high photosynthetic rates at high temperatures and intensive light, or at low temperatures and low light levels. Low temperatures at high light intensity may have a negative effect on their photosynthesis (Haraguchi and Yamada 2011) and may have been one of the reasons for low chlorophyll content observed in S. lindbergii in mid June 2008. Low chlorophyll content at the beginning of the season might also be due to active growth, when shoots are growing fast, and chlorophyll biosynthesis rates do not match those of shoot growth (Kershaw and Webber 1986). Later in the season, intensive light combined with high ambient temperature may explain the increase in total chlorophyll in S. lindbergii (Fig. 7).
Correlations (Pearson correlation coefficients) between the total chlorophyll concentration (Chl) of the three studied species and temperature and UVB on the three different dates the UVB was measured and samples collected
Chl in S. balticum
Chl in S. jensenii
Chl in S. lindbergii
Initially we hypothesized that Sphagnum mosses growing in the UVB-attenuation plots would have higher chlorophyll content as compared to the mosses in the ambient or control conditions. However, measurements revealed a higher chlorophyll concentration and chl a/b ratio in S. lindbergii sampled from ambient plots at the beginning of the season in 2008. This may be due to better overwintering abilities and higher photosynthetic activity in the plants in ambient conditions compared to those growing under increased temperatures (Gunnarsson et al. 2004). Higher total chlorophyll content in S. lindbergii in the control compared to −UVB plots on 3rd of July 2008 may also be explained by the higher UVA radiation doses under cellulose acetate filters.
At the end of the growing season in 2008, we detected no statistical differences between the treatments; instead, there were significant differences among the species in each treatment. This indicated that it was not the changes in temperature and UVB irradiance that affected the chlorophyll seasonality most, but rather species adaptability and their habitat requirements.
A slight temperature increase and/or a significant attenuation of solar UVB radiation (to less than 1 % of the ambient) had no clear effect on the chlorophyll concentration or its seasonal variation in the Sphagnum species growing in an open fen. Altogether, the species studied seemed to be adapted to the changes in the environment they were exposed to. Seasonal variation of chlorophyll concentration in Sphagnum mosses proved to be species-specific and may be linked to environmental requirements of the species. S. lindbergii, dominant on the experimental site, showed good adaptive capabilities, since it was able to sustain high chlorophyll concentration throughout the season irrespective of the treatment applied.
AH carried out the sampling, performed chemical analysis and drafted the manuscript, MT assisted in designing the experiment, participated in sampling and drafting the manuscript, PR performed the statistical analysis and assisted in drafting the manuscript, SH accomplished overall supervision and coordination of the research and helped to draft the manuscript. All authors read and approved the final manuscript.
The authors would like to express their gratitude to K. Lakkala and H. Suokanerva (Arctic Research Centre of Finnish Meteorological Institute, Sodankylä) for providing the UVB-BE and precipitation data, as well as the staff of the Finnish Forest Research Institute (Northern Unit, Rovaniemi), particularly J. Puoskari and H. Posio, for assistance in organizing the experiment, and F. Martz for developing the protocol for chlorophyll analysis. We also thank S. Ulich for proofreading the article. This research, as a part of ECOREIN project (2006–2009), was financially supported by the Thule Institute of the University of Oulu.
Compliance with ethical guidelines
Competing interests The authors declare that they have no competing interests.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
- Aphalo PJ (2003) Do current levels of UV-B radiation effect vegetation? The importance of long-term experiments. New Phytol 160:273–280View ArticleGoogle Scholar
- Baxter R, Emes MJ, Lee JA (1992) Effects of an experimentally applied increase in ammonium on growth and amino-acid metabolism of Sphagnum cuspidatum Ehrh. ex. Hoffm, from differently polluted areas. New Phytol 120:265–274View ArticleGoogle Scholar
- Björn LO, Callaghan TV, Gehrke C, Gwynn-Jones D, Lee JA, Johanson U, Sonesson M, Buck ND (1999) Effects of ozone depletion and increased ultraviolet-B radiation on northern vegetation. Polar Res 18:331–337View ArticleGoogle Scholar
- Bonnett SAF, Ostle N, Freeman C (2010) Short-term effect of deep shade and enhanced nitrogen supply on Sphagnum capillifolium morphophysiology. Plant Ecol 207:347–358View ArticleGoogle Scholar
- Breeuwer A, Heijmans MMPD, Robroek BJM, Berendse F (2008) The effect of temperature on growth and competition between Sphagnum species. Oecologia 156:155–167View ArticleGoogle Scholar
- Caldwell MM (1971) Solar UV irradiation and the growth development of higher plants. In: Giese C (ed) Photophysiology VI. Academic Press, New York, pp 131–268View ArticleGoogle Scholar
- Caldwell MM, Robberecht R, Billings WD (1980) A steep latitudinal gradient of solar ultraviolet-B radiation in the Arctic-Alpine life zone. Ecology 61:600–611View ArticleGoogle Scholar
- Caldwell MM, Bornman JF, Ballaré CL, Flint SD, Kulandaivelu G (2007) Terrestrial ecosystems, increased solar ultraviolet radiation, and interactions with other climate change factors. Photochem Photobiol Sci 6:252–266View ArticleGoogle Scholar
- Clymo RS, Hayward PM (1982) The ecology of Sphagnum. In: Smith AJE (ed) Bryophyte ecology. Chapman & Hall, London, pp 229–289View ArticleGoogle Scholar
- Csintalan Z, Tuba Z, Takács Z, Laitat E (2001) Responses of nine bryophyte and one lichen species from different microhabitats to elevated UV-B radiation. Photosynthetica 39:317–320View ArticleGoogle Scholar
- Dorrepaal E, Aerts R, Cornelissen JHC, Callaghan TV, van Logtestijn RSP (2003) Summer warming and increased winter snow cover affect Sphagnum fuscum growth, structure and production in a sub-arctic bog. Glob Chang Biol 10:93–104View ArticleGoogle Scholar
- Finnish Meteorological Institute (FMI) Database. Temperature and precipitation statistics from 1961 onwards. http://en.ilmatieteenlaitos.fi/statistics-from-1961-onwards. Accessed 31 Aug 2015
- Gaberščik A, Martinčič A (1987) Seasonal dynamics of net photosynthesis and productivity of Sphagnum papillosum. Lindbergia 13:105–110Google Scholar
- Gehrke C (1998) Effects of enhanced UV-B radiation on production-related properties of a Sphagnum fuscum dominated subarctic bog. Funct Ecol 12:940–947View ArticleGoogle Scholar
- Gerdol R (1996) The seasonal growth pattern of Sphagnum magellanicum Brid. in different microhabitats on a mire in the southern Alps (Italy). Oecol Mont 5:13–20Google Scholar
- Gerdol R, Bonora A, Poli F (1994) The vertical pattern of pigment concentrations in chloroplasts of Sphagnum capillifolium. Bryologist 97:158–161View ArticleGoogle Scholar
- Gignac LD (2011) Bryophytes as predictors of climate change. In: Tuba Z, Slack NG, Stark LR (eds) Bryophyte ecology and climate change. Cambridge University Press, New York, pp 461–482Google Scholar
- Glime JM (2007) Bryophyte ecology. http://www.bryoecol.mtu.edu/
- Gorham E (1991) Northern peatlands: role in the carbon cycle and probable responses to climatic warming. Ecol Appl 1:182–195View ArticleGoogle Scholar
- Gunnarsson U, Granberg G, Nilsson M (2004) Growth, production and interspecific competition in Sphagnum: effects of temperature, nitrogen and sulphur treatments on a boreal mire. New Phytol 163:349–359View ArticleGoogle Scholar
- Hájek T, Tuittila E-S, Ilomets M, Laiho R (2009) Light responses of mire mosses—a key to survival after water-level drawdown? Oikos 118:240–250View ArticleGoogle Scholar
- Haraguchi A, Yamada N (2011) Temperature dependency of photosynthesis of Sphagnum spp. distributed in the warm-temperate and the cool-temperate mires of Japan. Am J Plant Sci 2:716–725View ArticleGoogle Scholar
- Huttunen S, Lappalainen NM, Turunen J (2005) UV-absorbing compounds in subarctic herbarium bryophytes. Environ Pollut 133:303–314View ArticleGoogle Scholar
- Jylhä K, Tuomenvirta H, Ruosteenoja K (2004) Climate change projections for Finland during the 21st century. Boreal Environ Res 9:127–152Google Scholar
- Kershaw KA, Webber MR (1986) Seasonal changes in the chlorophyll content and quantum efficiency of the moss Brachythecium rutabulum. J Bryol 14:151–158View ArticleGoogle Scholar
- Laine J, Harju P, Timonen T, Laine A, Tuittila E-S, Minkkinen K, Vasander H (2009) The intricate beauty of Sphagnum mosses—a Finnish guide to identification. University of Helsinki, Department of Forest Ecology, HelsinkiGoogle Scholar
- Lappalainen NM, Huttunen S, Suokanerva H (2008) Acclimation of a pleurocarpous moss Pleurozium schreberi (Britt.) Mitt. to enhanced ultraviolet radiation in situ. Glob Chang Biol 14:321–333View ArticleGoogle Scholar
- Limpens J, Berendse F, Blodau C, Canadell JG, Freeman C, Holden J, Roulet N, Rydin H, Schaepman-Strub G (2008) Peatlands and the carbon cycle: from local processes to global implications—a synthesis. Biogeosci Discuss 5:1379–1419View ArticleGoogle Scholar
- Martin CE, Churchill SP (1982) Chlorophyll concentrations and a:b ratios in mosses collected from exposed and shaded habitats in Kansas. J Bryol 12:297–304View ArticleGoogle Scholar
- Martínez-Abaigar J, Núñiez-Olivera E (1998) Ecophysiology of photosynthetic pigments in aquatic bryophytes. In: Bates JW, Ashton NW, Duckett JG (eds) Bryology for the twenty-first century. Maney Publishing, Leeds, pp 277–292Google Scholar
- Martínez-Abaigar J, Núñiez-Olivera E, Sánchez-Díaz M (1994) Seasonal changes in photosynthetic pigment composition of aquatic bryophytes. J Bryol 18:97–113View ArticleGoogle Scholar
- Martz F, Sutinen M-L, Derome K, Wingsle G, Julkunen-Tiitto R, Turunen M (2007) Effects of ultraviolet (UV) exclusion on the seasonal concentration of photosynthetic and UV-screening pigments in Scots pine needles. Glob Chang Biol 13:252–265View ArticleGoogle Scholar
- Martz F, Turunen M, Julkunen-Tiitto R, Lakkala K, Sutinen M-L (2009) Effect of the temperature and the exclusion of UVB radiation on the phenolics and iridoids in Menyanthes trifoliata L. leaves in the subarctic. Environ Pollut 157:3471–3478View ArticleGoogle Scholar
- McBean G (2005) Arctic climate: past and present. In: Symon C, Arris L, Heal B (eds) Arctic climate impact assessment. Cambridge University Press, New York, pp 21–60Google Scholar
- Mishler BD, Oliver MJ (1991) Gametophytic phenology of Tortula ruralis, a desiccation-tolerant moss, in the organ mountains of southern New Mexico. Bryologist 94:143–153View ArticleGoogle Scholar
- Naumov AV, Kosykh NP (2011) The structure and functional features of Sphagnum cover of the Northern West Siberian mires in connection with forecasting global environmental and climatic changes. In: Tuba Z, Slack NG, Stark LR (eds) Bryophyte ecology and climate change. Cambridge University Press, New York, pp 299–315Google Scholar
- Niemi R, Martikainen PJ, Silvola J, Sonninen E, Wulff A, Holopainen T (2002a) Responses of two Sphagnum moss species and Eriophorum vaginatum to enhanced UV-B in a summer of low UV intensity. New Phytol 156:509–515View ArticleGoogle Scholar
- Niemi R, Martikainen PJ, Silvola J, Wulff A, Turtola S, Holopainen T (2002b) Elevated UV-B radiation alters fluxes of methane and carbon dioxide in peatland microcosms. Glob Chang Biol 8:361–371View ArticleGoogle Scholar
- Porra RJ, Thompson WA, Kriedemann PE (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochim Biophys Acta 975:384–394View ArticleGoogle Scholar
- Proctor MCF (2000) Physiological ecology. In: Shaw AJ, Goffinet B (eds) Bryophyte biology. Cambridge University Press, Cambridge, pp 225–247View ArticleGoogle Scholar
- Proctor MCF (2011) Climatic responses and limits of bryophytes: comparisons and contrasts with vascular plants. In: Tuba Z, Slack NG, Stark LR (eds) Bryophyte ecology and climate change. Cambridge University Press, New YorkGoogle Scholar
- Rinnan R, Saarnio S, Haapala JK, Mörsky SK, Martikainen PJ, Silvola J, Holopainen T (2013) Boreal peatland ecosystems under enhanced UV-B radiation and elevated tropospheric ozone concentration. Environ Exp Bot 90:43–52View ArticleGoogle Scholar
- Robson TM, Pancotto VA, Flint SD, Ballaré CL, Sala OE, Scopel AL, Caldwell MM (2003) Six years of solar UV-B manipulations affect growth of Sphagnum and vascular plants in a Tierra del Fuego peatland. New Phytol 160:379–389View ArticleGoogle Scholar
- Robson TM, Pancotto VA, Ballaré CL, Sala OE, Scopel AL, Caldwell MM (2004) Reduction of solar UV-B mediates changes in the Sphagnum capitulum microenvironment and the peatland microfungal community. Oecologia 140:480–490View ArticleGoogle Scholar
- Rozema J, Boelen P, Blokker P (2005) Depletion of stratospheric ozone over the Antarctic and Arctic: responses of plants of polar terrestrial ecosystems to enhanced UV-B, an overview. Environ Pollut 137:428–442View ArticleGoogle Scholar
- Rydin H, Jeglum J (2006) The biology of peatlands. Oxford University Press, New YorkView ArticleGoogle Scholar
- Rydin H, Gunnarsson U, Sundberg S (2006) The role of Sphagnum in peatland development and persistence. In: Wieder RK, Vitt DH (eds) Boreal peatland ecosystems. Springer, Berlin, pp 47–65View ArticleGoogle Scholar
- Sonesson M, Carlsson BÅ, Callaghan TV, Halling S, Björn LO, Bertgren M, Johanson U (2002) Growth of two peat-forming mosses in subarctic mires: species interactions and effects of simulated climate change. Oikos 99:151–160View ArticleGoogle Scholar
- Soppela P, Turunen M, Forbes B, Aikio P, Magga H, Sutinen, M-L, Lakkala K, Uhlig C (2006) The chemical response of reindeer summer pasture plants in a subarctic peatland to ultraviolet (UV) radiation. In: Forbes BC, Bölter M, Müller-Wille L, Hukkinen J, Müller F, Gunslay N, Konstantinov Y (eds) Reindeer management in northernmost Europe. Linking practical and scientific knowledge in social-ecological systems, ecological studies, vol. 184. Springer, Berlin, pp 199–213Google Scholar
- Vitt DH (2000) Peatlands: ecosystems dominated by bryophytes. In: Shaw AJ, Goffinet B (eds) Bryophyte biology. Cambridge University Press, Cambridge, pp 312–337View ArticleGoogle Scholar
- Weatherhead B, Tanskanen A, Stevermer A (2005) Ozone and ultraviolet radiation. In: Symon C, Arris L, Heal B (eds) Arctic climate impact assessment. Cambridge University Press, New York, pp 151–182Google Scholar
- Weltzin JF, Pastor J, Harth C, Bridgham SD, Updegraff K, Chapin CT (2000) Response of bog and fen plant communities to warming and water-table manipulations. Ecology 81:3464–3478View ArticleGoogle Scholar
- Wiedermann MM, Nordin A, Gunnarsson U, Nilsson MB, Ericson L (2007) Global change shifts vegetation and plant—parasite interactions in a boreal mire. Ecology 88:454–464View ArticleGoogle Scholar
- Wojtuń B, Sendyk A, Martyniak D (2013) Sphagnum species along environmental gradients in mires of the Sudety mountains (SW Poland). Boreal Environ Res 18:74–88Google Scholar
- Yefremov SP, Yefremova TT, Eфpeмoв CП, Eфpeмoвa TT (2000) Construction and productivity of a Sphagnum moss community on West Siberia mires. Sibirskiy Ekologicheskiy Zhurnal 5:615–626 [In Russian] Google Scholar