Fig. 3

Luteolin inhibits MPA-induced VEGF mRNA expression in T47-D breast cancer cells. T47-D cells were treated at 37 °C for 6 h with 10 nM MPA ± 25 µM luteolin (LU) or 1 µM RU-486 (RU), or 25 µM LU or 1 µM RU-486 alone, after which RNA was isolated and RT-PCR for VEGF isoforms performed. Upper panel Representative figure of PCR-amplified VEGF products, showing VEGF 189, 165, and 121 bp bands and the GAPDH band used for normalization. Lower panel Results represent mean band intensities (VEGF/GAPDH) ± SEM (n = 3). *Significantly different from control (DMSO only) (P < 0.001, ANOVA). **Significantly different from MPA (P < 0.001, ANOVA)