From: Establishment of a sticky, large, oval-shaped thrombocyte cell line from tree frog as an ancestor of mammalian megakaryocytes
Solution
No. of adhered cells/area (mean ± standard deviation)
10 % HS-αMEM (control)
0 ± 0
0.02 % EDTA-PBS
43.3 ± 6.9*
PBS
39.8 ± 9.6*
PBS + MgCl2 + CaCl2
10 % HS-PBS
2.8 ± 2.4
10 % HS-0.02 % EDTA-PBS
0.5 ± 1.0