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Table 1 Adhesion test

From: Establishment of a sticky, large, oval-shaped thrombocyte cell line from tree frog as an ancestor of mammalian megakaryocytes

Solution No. of adhered cells/area (mean ± standard deviation)
10 % HS-αMEM (control) 0 ± 0
0.02 % EDTA-PBS 43.3 ± 6.9*
PBS 39.8 ± 9.6*
PBS + MgCl2 + CaCl2 0 ± 0
10 % HS-PBS 2.8 ± 2.4
10 % HS-0.02 % EDTA-PBS 0.5 ± 1.0
  1. The FUHEN cells were washed two times with 1 % HS-αMEM, and were suspended with various solutions, respectively. Then the cells were seeded in four petri dishes (Falcon 1008) at a density of 3 × 104 cells/dish. After 5 min of incubation at room temperature, the dishes were washed two times with PBS and fixed with 70 % EtOH for 3 h. Then, the cells were stained with hematoxylin or May-Grünwald Giemsa, and photographs were taken. The number of attached cells per area was counted. One-way ANOVAs and multiple comparison tests (Tukey–Kramer’s HSD test) were carried out
  2. * Means significant (p < 0.05) difference compared to the control