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Fig. 6 | SpringerPlus

Fig. 6

From: Establishment of a sticky, large, oval-shaped thrombocyte cell line from tree frog as an ancestor of mammalian megakaryocytes

Fig. 6

cd41 expression in FUHEN cells. The partial coding sequence of cd41 (integrin alpha 2b) was cloned (NCBI accession no. LC027926). Degenerate PCR primers amplified a DNA fragment with a predicted size of 312 bp (a). The alignment of corresponding cd41 sequences in Hyla japonica (LC027926), Xenopus laevis (NM_001094754), Xenopus (Silurana) tropicalis (XM_012952892), Danio rerio (BC163305) and Mus musculus (BC137570) is indicated (b). The alignment was carried out using GENETYX-MAC software (Genetyx Co. Ltd., Tokyo, Japan). The cloned DNA fragment (262 bp) had 67 % homology to Xenopus laevis alpha 2b. The underlined amino acids indicate the integrin alpha superfamily domain

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