Fig. 3
Temperature-sensitive growth of FUHEN cells. Growth of FUHEN cells was analyzed at 16, 28 and 37 °C (a, b). Symbols indicate the culture temperature (circle 28 °C, square 37 °C, triangle 16 °C). The seeded cell number was either 7 × 104 cells/flask (a), or 7 × 105 cells/flask (b). The proliferating cells at 28 °C after 1 week (c) or 3 weeks (d). The surviving cells at 16 °C after 4 weeks are indicated (e, arrow head). The cells resumed proliferation when the temperature was shifted to 28 °C after 2 weeks (f)