From: Agrobacterium rhizogenes mediated hairy root induction in endangered Berberis aristata DC.
Explant | Infection period (h) | Co-cultivation with acetosyringone (48 h) | Co-cultivation without acetosyringone (48 h) | ||
---|---|---|---|---|---|
% transformation frequency | Days required for root induction | % transformation frequency | Days required for root induction | ||
Leaf | 0 | – | – | – | – |
1 | 30.55 ± 1.60b | 28.66 ± 0.51d | 21.46 ± 0.76b | 27.99 ± 0.38b | |
2 | 62.96 ± 2.45d | 26.22 ± 0.22c | 29.62 ± 2.44c | 27.66 ± 0.19b | |
Callus | 0 | – | – | – | – |
1 | 72.22 ± 1.60e | 24.33 ± 0.38b | 61.11 ± 1.60e | 27.66 ± 0.50b | |
2 | 54.63 ± 2.45c | 25.77 ± 0.39c | 36.11 ± 1.60d | 27.66 ± 0.88b |