Skip to main content

Table 3 Effect of acetosyringone (100 µM) on hairy root induction

From: Agrobacterium rhizogenes mediated hairy root induction in endangered Berberis aristata DC.

Explant Infection period (h) Co-cultivation with acetosyringone (48 h) Co-cultivation without acetosyringone (48 h)
% transformation frequency Days required for root induction % transformation frequency Days required for root induction
Leaf 0
1 30.55 ± 1.60b 28.66 ± 0.51d 21.46 ± 0.76b 27.99 ± 0.38b
2 62.96 ± 2.45d 26.22 ± 0.22c 29.62 ± 2.44c 27.66 ± 0.19b
Callus 0
1 72.22 ± 1.60e 24.33 ± 0.38b 61.11 ± 1.60e 27.66 ± 0.50b
2 54.63 ± 2.45c 25.77 ± 0.39c 36.11 ± 1.60d 27.66 ± 0.88b
  1. Values are the mean of  % transformation frequency ± SE at p = 0.05, determined by putting mean value of treatments to statistical package SPSS, with one way analysis of variance (ANOVA) and the statistical significance of results was measured using Duncan’s multiple range-posthoc test. The experiment was performed in triplicates. Value followed by same letter in a column is not significantly different. Data were recorded after 35 days. –: zero transformation frequency