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Table 3 Effect of acetosyringone (100 µM) on hairy root induction

From: Agrobacterium rhizogenes mediated hairy root induction in endangered Berberis aristata DC.

Explant

Infection period (h)

Co-cultivation with acetosyringone (48 h)

Co-cultivation without acetosyringone (48 h)

% transformation frequency

Days required for root induction

% transformation frequency

Days required for root induction

Leaf

0

1

30.55 ± 1.60b

28.66 ± 0.51d

21.46 ± 0.76b

27.99 ± 0.38b

2

62.96 ± 2.45d

26.22 ± 0.22c

29.62 ± 2.44c

27.66 ± 0.19b

Callus

0

1

72.22 ± 1.60e

24.33 ± 0.38b

61.11 ± 1.60e

27.66 ± 0.50b

2

54.63 ± 2.45c

25.77 ± 0.39c

36.11 ± 1.60d

27.66 ± 0.88b

  1. Values are the mean of  % transformation frequency ± SE at p = 0.05, determined by putting mean value of treatments to statistical package SPSS, with one way analysis of variance (ANOVA) and the statistical significance of results was measured using Duncan’s multiple range-posthoc test. The experiment was performed in triplicates. Value followed by same letter in a column is not significantly different. Data were recorded after 35 days. –: zero transformation frequency