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Table 2 Effect of different explants on hairy root induction

From: Agrobacterium rhizogenes mediated hairy root induction in endangered Berberis aristata DC.

Infection period (h) Co-cultivation period (h) % transformation frequency in
Leaf Nodal segment Callus
0.0 0.0
0.5 48 12.03 ± 0.92b 29.03 ± 2.03b
1 48 21.46 ± 0.76c 61.11 ± 1.60d
2 48 29.62 ± 2.44d 18.55 ± 0.94c 36.11 ± 1.60c
3 48 42.59 ± 0.92e 34.25 ± 0.92d 29.62 ± 2.44b
4 48 19.44 ± 1.60c 10.18 ± 0.92b
  1. Values are the mean of  % transformation frequency ± SE at p = 0.05, determined by putting mean value of treatments to statistical package SPSS, with one way analysis of variance (ANOVA) and the statistical significance of results was measured using Duncan’s multiple range-posthoc test. The experiment was performed in triplicates. Value followed by same letter in a column is not significantly different. Data were recorded after 45 days. –: zero transformation frequency