Agrobacterium rhizogenes mediated hairy root induction in endangered Berberis aristata DC.

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Table 2 Effect of different explants on hairy root induction

Infection period (h)	Co-cultivation period (h)	% transformation frequency in
Infection period (h)	Co-cultivation period (h)	Leaf	Nodal segment	Callus
0.0	0.0	–	–	–
0.5	48	12.03 ± 0.92^b	–	29.03 ± 2.03^b
1	48	21.46 ± 0.76^c	–	61.11 ± 1.60^d
2	48	29.62 ± 2.44^d	18.55 ± 0.94^c	36.11 ± 1.60^c
3	48	42.59 ± 0.92^e	34.25 ± 0.92^d	29.62 ± 2.44^b
4	48	19.44 ± 1.60^c	10.18 ± 0.92^b	–

Values are the mean of % transformation frequency ± SE at p = 0.05, determined by putting mean value of treatments to statistical package SPSS, with one way analysis of variance (ANOVA) and the statistical significance of results was measured using Duncan’s multiple range-posthoc test. The experiment was performed in triplicates. Value followed by same letter in a column is not significantly different. Data were recorded after 45 days. –: zero transformation frequency