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Table 1 Effect of infection and co-cultivation period on hairy root induction in leaves

From: Agrobacterium rhizogenes mediated hairy root induction in endangered Berberis aristata DC.

Infection period 24 h co-cultivation period 48 h co-cultivation period
Strain 532 Strain 2364 Strain 532 Strain 2364
% transformation frequency Days required for root induction % transformation frequency Days required for root induction % transformation frequency Days required for root induction % transformation frequency Days required for root induction
0
15 min
30 min 12.03 ± 0.92b 36.66 ± 0.66e
1 h 8.34 ± 0.01b 38.66 ± 0.66e 21.46 ± 0.76c 35.33 ± 0.33e
2 h 11.11 ± 2.77b 35.0 ± 0.57d 5.56 ± 2.78b 39 ± 0.57c 29.62 ± 2.44d 32.66 ± 1.45d 11.11 ± 2.77b 37 ± 0.58c
3 h 19.44 ± 2.77c 31.33 ± 0.33c 11.11 ± 2.77c 34.33 ± 0.88b 42.59 ± 0.92e 27.66 ± 0.33c 16.67 ± 0.01c 35 ± 0.57b
4 h 22.22 ± 2.77c 28.33 ± 0.33b 19.44 ± 1.60c 25.33 ± 0.33b
5 h
  1. Values are the mean of % transformation frequency ± SE at p = 0.05, determined by putting mean value of treatments to statistical package SPSS, with one way analysis of variance (ANOVA) and the statistical significance of results was measured using Duncan’s multiple range-Posthoc test. The experiment was performed in triplicates. Value followed by same letter in a column is not significantly different. Data were recorded after 45 days. –: zero transformation frequency