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Table 1 Effect of infection and co-cultivation period on hairy root induction in leaves

From: Agrobacterium rhizogenes mediated hairy root induction in endangered Berberis aristata DC.

Infection period

24 h co-cultivation period

48 h co-cultivation period

Strain 532

Strain 2364

Strain 532

Strain 2364

% transformation frequency

Days required for root induction

% transformation frequency

Days required for root induction

% transformation frequency

Days required for root induction

% transformation frequency

Days required for root induction

0

15 min

30 min

12.03 ± 0.92b

36.66 ± 0.66e

1 h

8.34 ± 0.01b

38.66 ± 0.66e

21.46 ± 0.76c

35.33 ± 0.33e

2 h

11.11 ± 2.77b

35.0 ± 0.57d

5.56 ± 2.78b

39 ± 0.57c

29.62 ± 2.44d

32.66 ± 1.45d

11.11 ± 2.77b

37 ± 0.58c

3 h

19.44 ± 2.77c

31.33 ± 0.33c

11.11 ± 2.77c

34.33 ± 0.88b

42.59 ± 0.92e

27.66 ± 0.33c

16.67 ± 0.01c

35 ± 0.57b

4 h

22.22 ± 2.77c

28.33 ± 0.33b

19.44 ± 1.60c

25.33 ± 0.33b

5 h

  1. Values are the mean of % transformation frequency ± SE at p = 0.05, determined by putting mean value of treatments to statistical package SPSS, with one way analysis of variance (ANOVA) and the statistical significance of results was measured using Duncan’s multiple range-Posthoc test. The experiment was performed in triplicates. Value followed by same letter in a column is not significantly different. Data were recorded after 45 days. –: zero transformation frequency