From: Agrobacterium rhizogenes mediated hairy root induction in endangered Berberis aristata DC.
Infection period | 24 h co-cultivation period | 48 h co-cultivation period | ||||||
---|---|---|---|---|---|---|---|---|
Strain 532 | Strain 2364 | Strain 532 | Strain 2364 | |||||
% transformation frequency | Days required for root induction | % transformation frequency | Days required for root induction | % transformation frequency | Days required for root induction | % transformation frequency | Days required for root induction | |
0 | – | – | – | – | – | – | – | – |
15 min | – | – | – | – | – | – | – | – |
30 min | – | – | – | – | 12.03 ± 0.92b | 36.66 ± 0.66e | – | – |
1 h | 8.34 ± 0.01b | 38.66 ± 0.66e | – | – | 21.46 ± 0.76c | 35.33 ± 0.33e | – | – |
2 h | 11.11 ± 2.77b | 35.0 ± 0.57d | 5.56 ± 2.78b | 39 ± 0.57c | 29.62 ± 2.44d | 32.66 ± 1.45d | 11.11 ± 2.77b | 37 ± 0.58c |
3 h | 19.44 ± 2.77c | 31.33 ± 0.33c | 11.11 ± 2.77c | 34.33 ± 0.88b | 42.59 ± 0.92e | 27.66 ± 0.33c | 16.67 ± 0.01c | 35 ± 0.57b |
4 h | 22.22 ± 2.77c | 28.33 ± 0.33b | – | – | 19.44 ± 1.60c | 25.33 ± 0.33b | – | – |
5 h | – | – | – | – | – | – | – | – |