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Table 3 Protein concentration (mg/L) and esterase activity (U/mg) of recombinant enzymes

From: Production of medium-chain volatile flavour esters in Pichia pastoris whole-cell biocatalysts with extracellular expression of Saccharomyces cerevisiae acyl-CoA:ethanol O-acyltransferase Eht1 or Eeb1

Sample

Protein concentration (mg/L)

Esterase activity (U/mg)a

Recombinant Eht1b

100.3 ± 10.3

74.5 ± 10.5

Recombinant Eeb1b

113.6 ± 9.7

81.6 ± 12.7

Controlc

7.7 ± 1.5

10.3 ± 0.7

  1. aEsterase activity was determined using p-nitrophenyl acetate as a substrate; protein yield was determined based on absorbance at 280 and 260 nm. Results are expressed as the mean ± standard deviation from triplicate experiments.
  2. bSample was collected from 120 h post-induction supernatant containing extracellular enzymes by recombinant P. pastoris.
  3. cSample was collected from 120 h post-induction supernatant by P. pastoris containing empty vector.
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