Table 1 Strains, plasmids and primers used in this study
Strain/plasmid/primer | Description | Source |
|---|---|---|
Strains | ||
E. coli Top 10 | A strain for high-efficiency cloning and plasmid propagation | TransGen Biotech, China |
P. pastoris GS115 | A methylotrophic yeast having a mutation in the histidinol dehydrogenase gene (his4) | TransGen Biotech, China |
Plasmids | ||
pGEM-T | A vector system for cloning of PCR products | Invitrogen, USA |
pPIC9 K | A P. pastoris expression vector carrying kanamycin resistance gene | Invitrogen, USA |
Primersa | ||
P1 | F: 5′-TTCCTAGGATGTCAGAAGTTTCCAAATG-3′ | Sangon Biotech, China |
R: 5′-TAGCGGCCGCGTGTGACATCATACGACTAA-3′ | ||
P2 | F: 5′-TTCCTAGGATGTTTCGCTCGGGT-3′ | Sangon Biotech, China |
R: 5′-ATGCGGCCGCAACATATTTATAAAACTAAC T-3′ | ||