Fig. 4

Normal cerebellar lamination and development in Sox3 knockout mice. A–D Sagittal sections of 14.5 dpc of the Sox3 null cerebellum. B Expression of GFP+ cells (green) in the ventricular germinal epithelium (VGE). GFP+ cells are absence in the external granular layer (EGL). C Higher magnification of the ventricular germinal epithelial cells shows coexpression of GFP and GLAST. D Higher magnification view of a GFP+ cell that coexpressed GLAST. Sagittal section of the Sox3 null cerebella at 18.5 dpc. E Sagittal section of 18.5 dpc of the Sox3 null cerebellum. F Pax6 + GCPs (red) populating the EGL indented to form the four principal fissures (asterisks: white = preculminate fissure; yellow = primary fissure; green = secondary fissure; blue = posterolateral fissure) of the 18.5 dpc cerebellum. G Calbindin+ Purkinje cell plates foliate simultaneously at the base of the principal fissures. H Sox3 knockout mice have a normal size and well patterned cerebellum. AB anterobasal lobe, AD anterodorsal lobe, C central lobe, P posterior lobe, I inferior lobe. Scale bar 100 µm (A–C; E–H); 50 µm (D).