A clinical protocol to inhibit the HGF/c-Met pathway for malignant mesothelioma with an intrapleural injection of adenoviruses expressing the NK4 gene

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Table 4 Expression of the NK4 gene in mice injected with Ad-NK4 into the pleural cavity

Tissues	Relative NK4 mRNA levels per 100 ng of synthesized cDNA
Tissues	Day 1	Day 7	Day 14
Brain	41.4 ± 8.0	2.4 ± 0.6	1.2 ± 0.3
Lung	21,563.1 ± 7,314.8	94.8 ± 37.9	10.5 ± 4.8
Stomach	244.9 ± 120.4	1.4 ± 0.4	6.2 ± 2.3
Spleen	111.6 ± 63.2	9.9 ± 2.9	3.6 ± 1.5
Kidney	13.7 ± 5.3	4.5 ± 2.4	1.2 ± 0.3
Liver	28.6 ± 2.8	25.3 ± 16.1	16.5 ± 5.4
Small intestine	34.6 ± 9.4	31.2 ± 2.0	9.3 ± 1.3
Colon	4.8 ± 1.5	1.7 ± 0.4	1.7 ± 0.4
Testis	2.8 ± 0.6	1.8 ± 0.2	0.5 ± 0.1

Data show average and SE values (n = 6). First-strand cDNA was amplified with the real time polymerase chain reaction and with the NK4 primers, forward 5′-CGAGGCCATGGTGCTATACTC-3′, and reverse 5′-TCAGCGCATGTTTTAATTGCA-3′, and the glyceraldehyde 3-phosphate dehydrogenase primers, forward 5′-AGCAAGGACACTGAGCAAGAG-3′, and reverse 5′-TATTATGGGGGTCTGGATG-3′, at 62°C for annealing and for 40 cycles. The NK4 mRNA amounts were estimated with the glyceraldehyde 3-phosphate dehydrogenase as a control and expressed as an arbitrary unit based on a standard sample.