Figure 3

MDA-MB-231 cell effects on H₂O₂ levels. H₂O₂ was measured in MEM plus and minus 10,000 MDA-MB-231 cells and plus and minus 1 mM sodium pyruvate. a MEM or b 10 μM compounds were co-incubated with 25 μM H₂O₂ substrate for 60 min at 37°C in a 5% CO₂ incubator. Measured luminescence is shown as relative light units (RLU) in part a. Measured luminescence was converted to concentration of H₂O₂ after background correction using a standard curve in part b. Data points are shown with error bars representing ± SEM. Data is representative of three separate experiments with triplicate determinations of each data point.