Figure 1

Structure of the Nnat locus in rabbits. The Nnat is located within the intron of Blcap and consists of three exons (white and black rectangular boxes) and two introns. The protein coding regions are indicated as black boxes. The gray boxes indicate Blcap. The black arrows indicate the transcription start sites of Nnat and Blcap. The box with red stripes within the promoter of Nnat represents the CpG island within the amplified region of BSP, which might be the DMR of the Nnat in rabbits. The circles indicate individual CpG dinucleotides within the BSP. Unmethylation (open) in the paternal-allele and methylation (closed) in the maternal-allele were presumed. Bisulfite-converted sequence of BSP products is shown with each CpG site marked in red; BstUI restriction sites (CGCG) are shown as black solid dots above the CpG sites and as bars below the circles.