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Table 2 Negative selection determination with 5-fluorocytosine

From: The codA gene as a negative selection marker in Citrus

5-FC selection (mg l−1)

Plant

NT

codA2

codA3

codA6

Regeneration (%)1

Shoots/explant2

Regeneration (%)1

Shoots/explan2

Regeneration (%)1

Shoots/explant2

Regeneration (%)1

Shoots/explant2

0

80 ± 4.93a

2.9 ± 0.52a

84 ± 6.08a

3.4 ± 0.65a

68 ± 12.50a

2.9 ± 0.32a

71 ± 11.71a

2.2 ± 0.40a

50

75 ± 8.02a

3.1 ± 0.52a

44 ± 10.39b

1.1 ± 0.49b

31 ± 10.01b

0.8 ± 0.40b

40 ± 13.74b

1.13 ± 0.25b

75

78 ± 2.08a

2.7 ± 0.40a

14 ± 7.50c

0.8 ± 0.35b,c

21 ± 9.16b

0.7 ± 0.21b,c

24 ± 11.53b

0.7 ± 0.28b

100

73 ± 6.11a

2.1 ± 1.10a,b

0c

0c

0c

0b,c

0c

0c

200

64 ± 6.05a

1.9 ± 0.46a,b

0c

0c

0c

0b,c

0c

0c

400

10 ± 7.09b

0.9 ± 0.7b

0c

0c

0c

0b,c

0c

0c

  1. Internodes from non-transgenic and three transgenic lines, carrying the codA::nptII gene, were placed in regeneration medium supplemented with various concentrations of 5-FC. Regeneration frequency and number of shoots per explants were taken after 70 days.
  2. Data are the means of three independent experiments. Values are regeneration % (1) and number of shoots per explants (2) ±standard deviation between repetitions. Values in a column followed by a common letter are not significantly different according to Tukey’s multiple range test (P < 0.05). Lines codA2, codA3, and codA6 have one, three, and two transgene cassette copies, respectively.