Figure 3
From: The codA gene as a negative selection marker in Citrus
Rooting of regenerated shoots from non-transgenic and codA transgenic internodes as a function of 5-FC selection. a Root regeneration was evaluated 45 days after the transfer of the shoots to rooting media. The bar chart shows the average frequency of root regeneration and the standard error of means for root induction using three independent experiments with a total of 30 shoots per treatment of non-transgenic and the codA2 transgenic line. The Y axis represents the average percentage of explants showing root growth. X axis reports the concentrations of 5-FC. b Demonstration of effects of 0 and 400 mg l−1 5-FC on Carrizo non-transgenic and transgenic line codA2 during rooting induction. Adventitious shoot from non-transgenic and codA2 transgenic line when placed on root-induction medium supplemented with various concentrations of 5-FC, as indicated in (a), after 45 days.