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Figure 4 | SpringerPlus

Figure 4

From: Estrogen and tamoxifen up-regulate FXYD3 on breast cancer cells: assessing the differential roles of ER α and ZEB1

Figure 4

Effect of decreased ZEB1 expression on FXYD3 expression. Decreasing ZEB1 expression was achieved by transfecting MCF-7 cells with siRNA targeting human ZEB1. Following the transfection procedure, cells were treated with control PBS/ethanol vehicle, or with estrogen, tamoxifen or with estrogen in combination with tamoxifen for 24 h. Expression of FXYD3 on cells was determined by flow cytometry using a Guava PC instrument and Guava Express software. Each bar represents the mean ± SD of FXYD3 fluorescence per cell for six to eight samples obtained in separate experiments. FXYD3 expression data from untransfected or mock transfected (non-targeting siRNA) cells were pooled together. Asterisk significant difference from ZEB1 siRNA transfected, control-treated cells.

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