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Figure 4 | SpringerPlus

Figure 4

From: Parathyroid-hormone-related protein signaling mechanisms in lung carcinoma growth inhibition

Figure 4

ERK2 knockdown and H1944 cell proliferation. a ERK2 siRNA (closed bars) knocked down expression of the target by 70–80% compared to NTC siRNA (open bars). The effect lasted at least 72 h. ERK1 was not affected by ERK2 knockdown. b H1944 cell proliferation was measured by MTS assay in 4 clones of PTHrP-positive cells and 3 clones of PTHrP-negative cells for 0–72 h after transient transfection with ERK2 siRNA or NTC oligonucleotides. The line segments in the upper graph connect points with and without ERK2 knockdown for the same clone and time point. ERK2 knockdown reduced proliferation over 72 h in 3 out of 4 PTHrP-positive H1944 cell clones. In contrast, ERK2 knockdown had no significant effect on proliferation in three PTHrP-negative clones. The lower graph presents averages ± standard errors, with open bars and closed bars representing transfection with NTC siRNA and ERK2 siRNA, respectively. Data were analyzed by paired t test comparing the effects of knockdown at a given time point between cells from the same clone. *P < 0.05, **P < 0.01 for NTC vs. ERK2 knockdown.

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