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Figure 6

From: Functional and expression analyses of two kinds of betaine aldehyde dehydrogenases in a glycinebetaine-hyperaccumulating graminaceous halophyte, Leymus chinensis

Figure 6

Expression pattern of mRNA and proteins of BADH and CMO-like in Leymus chinensis and barley plants under normal and saline conditions. Four-week-old L. chinensis and three-week-old barley plants grown hydroponically with same leaf stage (the youngest fully-expanded leaf was sixth from the bottom) were treated with 0 and 300 mM NaCl for 72 h. The sixth leaf blades and roots were harvested and used for the analyses. (a) Real-time PCR analysis of the transcript level of LcBADH1, LcBADH2 and CMO-like genes under normal and saline conditions in the sixth leaf blades. LcTubulin was used as an internal control to normalize for variation in the amount of cDNA template. Data show the mean of relative values with one unit being control (0 h) ± SD of three biological replicates. The symbols ** represent significant differences from control values at each treatment period at P < 0.01 (Student’s t-test). (b) Protein gel-blot analyses of BADH and CMO-like proteins. The lanes were loaded with protein extracted from sixth leaf blades and roots equivalent to 0.25 mg FW. The sizes of molecular mass standards are shown on the left in kDa.

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