Figure 7
Molecular characterizations of T0 and T1 progeny of FlAc7 and FlAc11 transgenic tomato plants. A Comparative real-time PCR analysis of transcript in T0 Flcry1Ac transgenic plants showing fold change in expression with respect to FlAc 9 (low expressing transgenic plant taken as reference). Control : non-transgenic control. B, C RT-PCR and cDNA amplification of 678 bp nptII gene and 768 bp Flcry1Ac gene of T1 progeny using specific primers. M : 100 bp DNA ladder (NEB, USA). -C : non-transgenic control plant, +C : Flcry1Ac gene plasmid DNA as positive control. D Southern blot probed with 3,510 bp BamHI radiolabelled frament of FlCry1Ac gene. E Western immunoblot assay performed with crude leaf protein extract, lane1 purified Cry1Ac toxin protein, lane 12 : untransformed control, lane 2–11 : leaf protein extracts from progeny of T0 FlAc7 and FlAc11. A protein band of ~130 kDa in transgenic plants showed hybridization with Cry1Ac antibodies, similar to positive control.