Figure 5

Molecular characterizations of T₀ Flcry1Ac transgenic tomato plants. Upper Panel A–C PCR amplification of 768 bp of Flcry1Ac gene using specific primers in thirty T₀ transgenics. D–F RT-PCR analysis of T₀ transgenic tomato plants showing 768 bp amplicon of cry1Ac gene. M : 100 bp DNA ladder (NEB, USA). -C : non-transgenic control plant, +C : plasmid DNA positive control. Lower Panel G–I PCR amplification of 678 bp nptII gene using specific primers in T₀ transgenics. J–L RT-PCR analysis of T₀ transgenic tomato plants of showing 678 bp amplicon of nptII gene. M : 100 bp DNA ladder (NEB, USA). -C : non-transgenic control plant, +C : plasmid DNA positive control.