Figure 3
Southern blot hybridisation and Western immunoblot analysis of T0 Trcry1Ac transgenics. A Southern blot hybridisation analysis of eight T0 transgenic tomato plants of cry1Ac probed with 1,845 bp radiolabelled BamHI-EcoRI fragment of cry1Ac gene. +C 1,845 bp cry1Ac gene fragment; −C Untransformed control plant. B Western immunoblot analysis of ten T0 transgenic plants, with crude protein extract; lane 1: purified Cry1Ac protein; lane 12 : untransformed control plant protein; lane 2–11: protein samples of ten transgenic plants. The transgenic samples gave a band of ~65 kDa, similar to the positive control. –C : Untransformed control plant.