Figure 2

Screening of T₀ and T₁ Trcry1Ac transgenic tomato plants. A–C PCR amplification of 995 bp of cry1Ac gene. D–F 678 bp of nptII gene using gene specific primers in thirty T₀ transgenics. G, H RT-PCR analysis of ten randomly selected T₀ transgenic tomato plants of cry1Ac showing 995 bp cry1Ac gene and 678 bp nptII gene transcripts. M – 100 bp DNA ladder (NEB, USA). -C – non-transgenic control plant, +C – plasmid DNA positive control. I Real-time analysis for Bt-cry1Ac transcript levels in six T₀ transgenic tomato plants. TC – Transgenic plant with low expression of Bt-toxin used as control. J–O PCR amplification of 995 bp of cry1Ac gene and 678 bp of nptII gene using gene specific primers inT₁ progeny of promising T₀ parents. M −100 bp DNA ladder (NEB, USA). –C : non-transgenic control plant, +C : plasmid DNA positive control.