Figure 1

Nucleotide sequences of the region encompassing reiteration VII of HSV-1. (a) Nucleotide sequences of strain 17 (as the standard) and isolate Ty2. The nucleotide numbering system used is the short unique region of strain 17 (McGeoch et al. 1985). Nucleotide sequences recognized by restriction endonuclease BamHI were single underlined. Each copy of the 18-bp tandem repeats of reiteration VII was underlined by a broken line having an arrowhead at both ends. Three copies of the 18-bp repeats plus 6-bp of the partial copy of reiteration VII were present in strain 17. A partial copy of 15-bp was present in reiteration VII of isolate Ty2, and no complete copy of the 18-bp repeats was present in isolate Ty2. A deletion of 45-bp (nucleotide no. 12219–12263) (del-2) found in the DNA of isolate Ty2 relative to that of strain 17 was indicated by a broken line. A duplication of 18-bp (nucleotide no. 12215–12218, and 12264–12277) (mul-2) was found in the DNA of isolate Ty2 relative to that of strain 17, and each copy of 18-bp of the duplication was indicated by a solid line having a closed circle at both ends. Each nucleotide of isolate Ty2 that differed from the corresponding nucleotide of strain 17 was enclosed within a rectangle. (b) Nucleotide sequences of strain 17 involved in the generation of deletions and multiplications. In the present study, six kinds of deletions (del-1 to del-6) and four kinds of multiplications (mul-1 to mul-4) were identified (Table 3). Each pair of direct repeats, which was thought to be involved in the generation of deletions and multiplications, was indicated by a pair of solid lines, each of which had an arrowhead at both ends.