Figure 7

Detection of the hydroxyl radicals produced in E. coli treated by EDF under ampicillin stress. The method used was similar to that described in Figure 4. The final concentrations of ampicillin (Amp) and HPF were 15 μg/mL and 5 μg/mL, respectively. After the incubation at 37°C for 4 hours without shaking, cells were collected and fluorescence density was analyzed on a flow cytometer.