Binding of the ExpL3-myc fusion protein to microcrystalline cellulose (Avicel). The left panel shows the results of the Western blot analysis probed with the anti-c-Myc antibody 9E10. The right panel shows Coomassie brilliant blue R-250 (CBB) staining after SDS-PAGE using the same samples utilized in the Western analysis. The samples in each lane are: Total, input total cell lysate protein from the slug of act15:expL3 myc
OE strain; Unbound, Avicel unbound flow-through fraction; and Bound, Avicel bound fraction. The arrow indicates the position of the ExpL3-myc fusion protein.