Validation of endogenous reference genes in Buglossoides arvensis for normalizing RT-qPCR-based gene expression data

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Table 4 Comprehensive ranking of the ten candidate reference genes using geNorm and NormFinder algorithms

Experimental sample sets	Gene expression stability
Experimental sample sets	Most stable Least stable
Leaves	GAPDH, CAC, UBQ, α-tub, PP2a, RUBISCO, α-actin, EF-1a, 18S rRNA, β-actin
Stems	CAC, RUBISCO, α-tub, β-actin, UBQ, α-actin, PP2a, EF-1a, GAPDH, 18S rRNA
Leaves + PGPR	α-tub, GAPDH, EF-1a, PP2a, CAC, UBQ, 18S rRNA, β-actin, RUBISCO, α-actin
Seeds / Flowers	α-tub, CAC, UBQ, GAPDH, α-actin, PP2a, RUBISCO, β-actin, 18S rRNA, EF-1a
Temperature	α-tub, GAPDH, CAC, UBQ, PP2a, α-actin, 18S rRNA, β-actin, EF-1a, RUBISCO
Total Samples	CAC, α-tub, UBQ, PP2a, GAPDH, β-actin, RUBISCO, 18S rRNA, EF-1a, α-actin