Table 2 TaqMan-based RT-qPCR primer and probes designed for the ten reference genes isolated from B. arvensis
Gene symbol | Primer and Probe Sequences | B. arvensis GenBank Accession no. | Amplification efficiency (%) | r 2 |
|---|---|---|---|---|
Complete description | (5´ → 3´) | |||
β-actin | βAct-F : CCGTCGGGCAACTCATAGTT | KJ883538 | 94.5 | 0.998 |
Beta-Actin | βAct-R : GCAGGAGCTTGACACTTCCAA | |||
FAM-TTCTCAATTGATGAGCTGCT-MGBNFQ | ||||
18S rRNA | 18S-F: TCACGACCCGGCCAATT | KJ883536 | 97.8 | 0.997 |
18S ribosomal RNA | 18S-R: CCGATCGTCTCGTCTCTTCTG | |||
FAM-AGGCCAGGAGCGTAT-MGBNFQ | ||||
EF-1a | EF1a-F: GCCACACCATCACCAGATACAT | KJ883544 | 91.5 | 0.986 |
Elongation factor 1a | EF1a-R: CGTTTTGATGGATTGAGTGATACTG | |||
FAM-ACTGCAAAATAAATCGAC-MGBNFQ | ||||
α-tub | TUB-F: CCTGAGAAACAAGCCTGTTGAGA | KJ920355 | 100.2 | 0.999 |
Alpha tubulin | ||||
TUB-R: CGCAAGTCCCTCGACATTG | ||||
FAM-TGGTGTAAGTTGGGCGC-MGBNFQ | ||||
UBQ | UBQ-F: CTTCCCGGTGAGGGTCTTG | KJ883543 | 103.2 | 0.998 |
Ubiquitin | UBQ-R: ACTTGGTGCTCAGGCTTCGT | |||
FAM-CGAAGATCTGCATACCAC-MGBNFQ | ||||
CAC | CAC-F: TTGACTGTTTGGTTTGGAAGATAAGA | KJ883539 | 101.6 | 0.995 |
Clathrin adaptor complexes | CAC-R: TTCCACCTCAGCACTCAATGTAG | |||
FAM-AATTTCCTGGACAAACTG -MGBNFQ | ||||
GAPDH | GAPDH-F: AATGGAAGCACAATGAACTTAAGGT | KJ883540 | 99.7 | 0.990 |
Glyceraldehyde-3-phosphate dehydrogenase | GAPDH-R: CGTACTGGTTTTTCTCCGAAGAG | |||
FAM-CATGATGAGAAGACCC-MGBNFQ | ||||
PP2a | PP2a-F: TGCCTTGGCTTCTGTTATTATGG | KJ883542 | 96.8 | 0.997 |
Protein phosphatase 2A regulatory subunit A | PP2a-R: GCTGTTCGATTGTAGCATCCTTT | |||
FAM-AATGGCCCCTGTTCTA-MGBNFQ | ||||
α- actin | AACT-F: CAAGGCTAACAGGGAGAAAATGA | KJ883537 | 104.6 | 0.995 |
Alpha-actin | AACT-R: TGAATAGCAACATACATAGCAGGAACA | |||
FAM-CAAATCATGTTTGAGACATT-MGBNFQ | ||||
RUBISCO | RUB-F: AGAATATTGGTGCTAAGTTGGTCAGA | KJ883541 | 97.9 | 0.994 |
Ribulose-1,5-bisphosphate carboxylase oxygenase | RUB-R: CCATCCCCAGCCAGATCAT | |||
FAM-AGCAGCATCAAAGAC-MGBNFQ |