Table 3 Sequences of the nucleotide primers used in this study
From: Characterization of aflatoxin producing Aspergillus flavus from food and feed samples
Primer code | Target gene | Primer sequences | Product size (bp) |
|---|---|---|---|
AflR-1for | aflR | 5′-AAGCTCCGGGATAGCTGTA-3′ | 1079 |
AflR-2rev | 5′-AGGCCACTAAACCCGAGTA-3′ | ||
AflS-1for | aflS (aflJ) | 5′-TGAATCCGTACCCTTTGAGG-3′ | 684 |
AflS-2rev | 5′-GGAATGGGATGGAGATGAGA-3′ | ||
AflD-1for | aflD (nor-1) | 5′-CACTTAGCCATCACGGTCA-3′ | 852 |
AflD-2rev | 5′-GAGTTGAGATCCATCCGTG-3′ | ||
AflM-1for | aflM (ver-1) | 5′-AAGTTAATGGCGGAGACG-3′ | 470 |
AflM-2rev | 5′-TCTACCTGCTCATCGGTGA-3′ | ||
AflO-1for | aflO (omtB) | 5′-TCCAGAACAGACGATGTGG-3′ | 790 |
AflO-2rev | 5′-CGTTGGCTAGAGTTTGAGG-3′ | ||
AflP-1for | aflP (omtA) | 5′-AGCCCCGAAGACCATAAAC-3′ | 870 |
AflP-2rev | 5′-CCGAATGTCATGCTCCATC-3′ | ||
AflQ-1for | aflQ (ordA) | 5′-TCGTCCTTCCATCCTCTTG-3′ | 757 |
AflQ-2rev | 5′-ATGTGAGTAGCATCGGCATTC-3′ |