From: Genetics, structure, and prevalence of FP967 (CDC Triffid) T-DNA in flax
Primer/probe name | Sequence | Tm | Notes |
---|---|---|---|
SAD reference: Amplifies 68 bp of stearoyl-acyl carrier protein desaturase | |||
P1 | GCTCAACCCAGTCACCACCT | 63 | |
P2 | TGCGAGGAGATCTGGAGGAG | 61 | |
prb1 | TGTTGAGGGAGCGTGTTGAAGGGA | 68 | |
Construct specific assay: Used by commercial testing companies to detect 105 bp of FP967 TDNA | |||
P3 | AGCGCGCAAACTAGGATAAA | 52 | In NOS 3’terminator |
P4 | ACCTTCCGGCTCGATGTCTA | 55 | In DHFR gene of E.coli SpecR cassette |
prb2 | CGCGCGCGGTGTCATCTATG | 67 | |
Event Specific assay: Detects WT gDNA at scaffold261 or the FP967 TDNA RB-flanking scaffold86 junction | |||
P13 | CTATCGTCTGACTCTGACTG | 49 | FFS1 flax genomic region |
P14 | CAACGCCCACTCTCTTTCTTA | 52 | FFS2 flax genomic region |
P15 | CCCTTAATTCTCCGCTCATGATCAG | 50 | RB region of TDNA |
prb3 | CACTTCTTCAATTTTATTTCAATATGTCTTTC | 60 | Specific for FP967 TDNA insertion site on scaffold86 |
prb5 | ATCAAACACTGATAGTTTAAACTGAAGGCGGG | 67 | Specific for RB region of TDNA |
Event specific assay SAT2-LB: Detects internal inverted repeat of E. coli SpecR cassette adjacent to LB | |||
P85 | TACATTAAAAACGTCCGCAATGTG | 59 | Located in LB |
P86 | CCTGCTCAGGGATCACCGAA | 62 | In SAT of E.Coli SpecR cassette |
prb28 | TATCCTGCCAAAAGCCGCGCCA | 68 | LB/Spec junction |