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Table 1 Use of the construct and event specific event assays to detect the FP967 TDNA

From: Genetics, structure, and prevalence of FP967 (CDC Triffid) T-DNA in flax

 

Norlin

FP967 TDNA

Hemizygote

Construct specific assay

   

P3 + P4 + probe2

 

X

X

P1 + P2 + probe1 (detects SAD reference gene)

X

X

X

Event specific assay

   

P13 + P14 + probe3

X

 

X

P13 + P15 + probe 5 and P14 + P15 + probe 5

 

X

X

  1. The construct specific assay is able to distinguish between WT and transgenic plants, while the event specific assay can also distinguish between hemizygous and homozygous individuals. Sequences of the primers and probes are shown in Table 2, while their approximate location is shown on Figure 2.
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