Expression and functional validation of heat-labile enterotoxin B (LTB) and cholera toxin B (CTB) subunits in transgenic rice (Oryza sativa)

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Table 1 Characteristics of transgenic rice lines

Event ID		IPCR prod.	Rice Seq.	No Chr.	Chr. From ^#	Chr. To ^#	T-DNA end	Adaptor start	Copy number	Gene insert
LCI-1	L*	302	302	3	35,147,954	35,147,653	−1	303	2	n.d
	R*	482	405	5	24,625,335	24,624,931	72	483
LCI-3	R	542	536	8	26,724,580	26,724,045	−1	−1	1	intron
LCI-4	R	623	552	7	4,833,382	4,832,831	73	624	2	n.d
LCI-5	L	149	150	3	31,727,379	31,727,528	−1	150	1	exon
	R	448	343	3	31,727,348	31,727,006	67	449
LCI-6	R	704	639	3	30,243,945	30,243,307	61	−1	1	intron
LCI-7	L	199	126	6	20,057,801	20,057,926	73	238	1	intergene
LCI-8	L	210	197	3	30,990,975	30,991,171	14	211	2	n.d
	R	347	275	9	17,404,938	17,405,212	72	348
LCI-10	R	214	64	2	46,488	46,425			1	intergene
LCI-11	L	318	318	12	798,649	798,332	−1	319	1	intergene
	R	590	521	12	798,672	799,192	72	591
LCI-13	L	395	395	9	16,474,041	16,474,435	−1	396	2	n.d
	R	211	184	5	24,829,622	24,829,805	25	212
LCI-17	L	684	681	3	31,691,652	31,690,972	−1	−1	1	intron
	R	546	437	3	31,691,676	31,692,112	70	547
LCI-18	R	217	146	1	32,792,438	32,792,293	72	218	1	intron

*L and R, left and right border sequences, respectively.
^#Rice Annotation Project Database (RAP-DB) was used to retrieve rice annotation information.
N.d., not determined.