Figure 6

Levels of LTB- and CTB-specific IgG and IgA in mouse serum and feces. (a) CTB- and LTB-specific systemic IgG and mucosal IgA in mouse serum following intraperitoneal injection of wild-type (WT) and LCI-11 seeds and Ed coli-expressed LTB and CTB proteins. (b) LTB- and CTB-specific systemic IgG and mucosal IgA in mouse serum fed orally with wild-type and transgenic rice seeds. PBS was used as the negative control. Western blot (inset) detection of LTB- and CTB-specific IgG and IgA in mice immunized orally with LCI-11. First lane, BSA; second lane, LTB or CTB proteins, respectively. (c) LTB-specific mucosal IgA levels in mouse feces fed orally with recombinant LTB protein, wild-type and transgenic rice seeds. PBS was used as the negative control.