Figure 5
Recombinant LTB and CTB protein expression by transgenic rice seeds. (a) GM1-ganglioside receptor binding assay of LTB and CTB protein expression in transgenic rice. The relative amount of LTB and CTB proteins present in LCI-11 was estimated by standard curves of recombinant LTB and CTB proteins, respectively. Arrows indicate relative amount of LTB and CTB proteins in LCI-11. BSA, bovine serum albumin. (b) Western blot analysis of LTB and CTB expression by transgenic rice seeds using LTB- (left) and CTB- (right) specific antibodies, respectively. Lane 1: positive control. Lane 2: wild-type rice. Lane 3: transgenic rice. Arrows indicate LTB and CTB proteins.