Molecular analyses of transgenic plants by PCR and real-time PCR in the LCI-7 and LCI-11 lines (T
generations). (a) Genomic DNA isolated from T3 to T5 transgenic plants of LCI-7 (left) and LCI-11 (right) lines was used and the bar, LTB, and CTB genes were amplified by PCR. Lane 1: 1 kb marker: Lane 2: wild-type rice: Lane 3, 4 and 5: T3–5 transgenic rice. (b) The LTB, CTB, and bar gene, and Tnos, copy numbers were analyzed in the T3, T4 and T5 generations of the LCI-11 rice line by real-time PCR.