Table 1 Primer sequences and product sizes for mtDNA 4977 bp deletion analysis in this study
Primer | Primer sequence | Position | Product | Note |
|---|---|---|---|---|
mtDNA-forward | 5′-GACGCCATAAAACTCTTCAC-3′ | 3457-3476 | 433 bp | ND1-region |
mtDNA-reverse | 5′-GGTTGGTCTCTGCTAGTGTG-3′ | 3889-3870 | ||
4977-1forward | 5′-TCAATGCTCGAAATCTGTGG-3′ | 8167-8187 | 496 bp | First PCR |
4977-1reverse | 5′-GTTGACCTGTTAGGGTGAGAAG-3′ | 13639-13618 | ||
4977-2forward | 5′-ACAGTTTCATGCCCATCGTC-3′ | 8196-8215 | 381 bp | Second PCR |
4977-2reverse | 5′-GCGTTTGTGTATGATATGTTTGC-3′ | 13553-13531 | ||
10398-forward | 5′-CCTGCCACTAATAGTTATGTC-3′ | 10307-10327 | 246 bp | ND3-region |
10398-reverse | 5′-GATATGAGGTGTGAGCGATA-3′ | 10552-10533 |