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Table 1 Effects of diet and weight classification on amino acid concentrations (nmol/ml) in the rat plasma

From: Rapid publication-ready MS-Word tables for two-way ANOVA

Variable

HF

LF

P -value

Lean

Overweight

Lean

Overweight

Diet

Weight

D × W 1

Asp

45.3 ± 2.39

47.6 ± 2.97

46.2 ± 2.96

45.3 ± 3.45

0.824

0.824

0.604

Glu

88.4 ± 3.23

88.6 ± 1.16

87.8 ± 2.58

87.6 ± 3.18

0.757

0.984

0.951

Asn

128 ± 5.71

114 ± 10.4

120 ± 5.62

133 ± 6.58

0.477

0.922

0.068

Ser

359 ± 10.3a

294 ± 4.39b

353 ± 7.43a

292 ± 3.76b

0.576

<0.001

0.807

Gln

562 ± 18.3b

645 ± 11.1a

559 ± 9.43b

655 ± 19.6a

0.801

<0.001

0.676

His

124 ± 3.18

115 ± 2.95

120 ± 4.08

130 ± 5.89

0.236

0.906

0.028

Gly

392 ± 6.91a

305 ± 7.3b

384 ± 7.32a

297 ± 5.68b

0.254

<0.001

0.955

Thr

379 ± 7.42

359 ± 11.6

381 ± 8.62

376 ± 12.6

0.365

0.223

0.481

Cit

79.9 ± 2.66a

53.8 ± 1.63c

69.6 ± 3.37b

73.9 ± 1.84ab

0.057

<0.001

<0.001

Arg

251 ± 8.09b

197 ± 6.4c

279 ± 7.46a

219 ± 4.46c

0.001

<0.001

0.642

β-Ala

13.6 ± 0.884b

31.4 ± 1.52a

13.1 ± 0.611b

26.8 ± 1.91a

0.064

<0.001

0.123

Taurine

648 ± 17.2a

469 ± 13.1c

670 ± 11.9a

572 ± 12b

<0.001

<0.001

0.006

Ala

471 ± 10.2b

403 ± 13.6c

492 ± 7.54b

585 ± 9.22a

<0.001

0.252

<0.001

Tyr

131 ± 4.25

135 ± 4.04

125 ± 5.7

139 ± 5.32

0.865

0.069

0.268

Trp

110 ± 3.28

114 ± 4.78

115 ± 2.61

113 ± 3.4

0.658

0.703

0.42

Met

108 ± 5.39

112 ± 3.65

107 ± 4.83

110 ± 4.8

0.77

0.521

0.991

Val

253 ± 9.61c

331 ± 6.74a

249 ± 10.1c

289 ± 9.63b

0.017

<0.001

0.048

Phe

111 ± 3.7

108 ± 3.84

106 ± 3.81

113 ± 3.44

0.988

0.645

0.211

Ile

152 ± 4.19b

206 ± 4.01a

144 ± 4.77b

195 ± 3.68a

0.035

<0.001

0.674

Leu

212 ± 4.56b

308 ± 7.56a

216 ± 6.34b

302 ± 5.79a

0.83

<0.001

0.465

Orn

68.8 ± 1.58b

81.4 ± 1.51a

67.8 ± 2.06b

83.3 ± 1.25a

0.786

<0.001

0.381

Pro

277 ± 7.04b

342 ± 8.59a

286 ± 5.32b

334 ± 4.52a

0.96

<0.001

0.196

Cys

165 ± 4.53b

197 ± 7a

157 ± 3.2b

194 ± 6.58a

0.279

<0.001

0.648

Lys

252 ± 8.82c

291 ± 5.17a

259 ± 8.32bc

282 ± 5.42ab

0.957

<0.001

0.273

  1. Values are means ± SEM, n = 9 per treatment group. Male Sprague-Dawley rats (Charles River Laboratories) were fed a low-fat (LF) or high-fat (HF) diet between 4 and 13 weeks of age, as described by Jobgen et al. (2009a, b). At 13 weeks of age, five hours after the last feeding, blood samples were obtained from the tail vein of box-restrained conscious rats using a microhematocrit (Wu 1995). The plasma was analyzed for amino acids using high-performance liquid chromatography (Rezaei et al. 2013; Wu and Meininger 2008). Classification of rats as lean or overweight was performed using the Cluster analysis of body weights, as described by Assaad et al. (2014b).
  2. a-cMeans in a row without a common superscript letter differ (P < 0.05) as analyzed by two-way ANOVA and the TUKEY test. 1D × W = Diet × Weight interaction effect.