Volume 4 Supplement 1

European Society for Neurochemistry Biannual Conference: Molecular Mechanisms of Regulation in the Nervous System

Open Access

Survival of retinal ganglion cells in transgenic mice with deficiencies in sialyltransferases or neural cell adhesion molecule (NCAM) or after the administration of neuraminidase

  • Natalia Lobanovskaya1,
  • Tamara Zharkovsky1,
  • Külli Jaako1,
  • Monika Jürgenson1,
  • Anu Aonurm-Helm1 and
  • Alexander Zharkovsky1
SpringerPlus20154(Suppl 1):P24

https://doi.org/10.1186/2193-1801-4-S1-P24

Published: 12 June 2015

Keywords

PSA-NCAMSialyltransferaseRetinal ganglion cell

Neural cell adhesion molecule (NCAM) plays important roles in the regulation of the brain plasticity during its development and in adulthood. NCAM functions may be regulated by the addition of long linear homopolymers of alpha 2-8-linked sialic acid (PSA). PSA is attached to NCAM via either of two specific sialyltransferases: ST8SiaII and ST8SiaIV. PSA-NCAM is expressed abundantly in the retina and optic nerve during development and adulthood. In the retina PSA-NCAM is expressed in the glial cells in close proximity to retinal ganglion cell (RGC). The functions of the PSA-NCAM in the retina remain unknown. The aim of this study was to investigate the roles of PSA-NCAM in the survival of RGCs after administration of the exitotoxin kainic acid (KA). Intraocular administration of KA induced reduction in the density of RGCs approximately by 60%. Administration of endoneuraminidase (Endo-N) an enzyme, which removes PSA residues from the surface of NCAM, enhanced the toxic effect of KA on RGC. In knockout mice with constitutive deficiency of either ST8SiaII or ST8SiaIV genes, the levels of PSA-NCAM did not differ from those in wild type mice. The toxicity of KA on RGC in these animals also did not differ from control. It should be noted, however, that in knockout ST8SiaII-/- adult mice a reduced number of RGCs was found despite the presence of high levels of PSA-NCAM. These data suggest that during development ST8SiaII ensures high levels of PSA-NCAM, which necessary for the developmental survival of RGCs. The PSA-NCAM in the adult retina ensures the resistance of RGCs to injury.

Declarations

Acknowledgements

This study was supported by the Estonian Science Council Grant (Institution research founding) IUT23, the Archimedes Foundation and the European Regional Development Fund. The authors thank Drs. H. Eckhardt and R. Gerardy-Schahn for their generous gift of transgenic animals.

Authors’ Affiliations

(1)
University of Tartu

Copyright

© Lobanovskaya et al. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.