Volume 4 Supplement 1

European Society for Neurochemistry Biannual Conference: Molecular Mechanisms of Regulation in the Nervous System

Open Access

Neurochemical changes in different brain regions induced by PACAP - relations to neuroprotection

  • Dora Reglodi1,
  • Gabor Maasz2,
  • Zsolt Pirger2,
  • Adam Rivnyak1,
  • Dorottya Balogh1,
  • Adel Jungling1,
  • Balazs Fulop1,
  • Laszlo Mark1 and
  • Andrea Tamas1
SpringerPlus20154(Suppl 1):L56

https://doi.org/10.1186/2193-1801-4-S1-L56

Published: 12 June 2015

Keywords

PACAP neuroprotection proteomics

Pituitary adenylate cyclase activating polypeptide (PACAP) is a neuropeptide with diverse occurrence and functions. One of the most well-known effects of PACAP is its strong neuroprotective effect. In this presentation we give an insight into recently described neurochemical changes induced by PACAP or altered by PACAP the lack of it. In an invertebrate model for Parkinson’s disease we found that PACAP effectively counteracts the dopamine-decreasing effect of rotenone, a mitochondrial neurotoxin. Similarly, in a 6-hydroxydopamine-induced rat model of Parkinson’s disease, we found that PACAP effectively increases dopamine levels. Furthermore, our proteomics analysis shows that PACAP treatment also counteracts the 6-OHDA-induced decrease in PARK-7 protein, effective against oxidative stress. Studying the role of endogenous PACAP, we found that PACAP-deficient mice show higher susceptibility to toxic agents causing degeneration of the substantia nigra dopaminergic neurons. Using proteomic analysis we revealed that the expression of numerous proteins is altered in the mesencephalon and striatum of knockout mice. Among the altered proteins, several are involved in metabolic processes, energy homeostasis, and structural integrity. ATP-synthase and tubulin beta-2A were expressed more strongly in PACAP-knockout mice. In contrast, the expression of more peptides/proteins markedly decreased in knockout mice, like pyruvate kinase, fructose biphosphate aldolase-A, glutathione S-transferase, peptidyl propyl cis-trans isomerase-A, gamma enolase, beta-synuclein and aspartate amino transferase. The altered expression of these proteins might partially account for the decreased antioxidant, cytoprotective and detoxifying capacity of PACAP-deficient mice. The described changes may provide further explanation for the neuroprotective potency of PACAP.

Declarations

Acknowledgements

OTKAK104984, Arimura Foundation, MTA-PTE Lendulet Program, KTIA_13_NAP-A-III/5, NAP-B.

Authors’ Affiliations

(1)
Department of Anatomy, MTA-PTE “Lendület” PACAP Research Team, University of Pecs
(2)
Centre for Ecological Research, HAS, Balaton Limnological Institute, Department of Experimental Zoology, Group of Chemical Ecology and Neurobiology

Copyright

© Reglodi et al. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.