Effects of mutations and deletions in the human optineurin gene

SpringerPlus

Table 1 Primer sequences

Expression vector	Sense primer	Antisense primer
pOPTN_R96L-EGFP	AAAGAAGCAAAAGAGCT TCTAATGGCCTTG	GCTCTTTTGCTTCTTTGCTCTGTATCTC
pOPTN_D474N-EGFP	CAGATGGAAGTTTACTGTTCTA ATTTTCATGCTGAAA GAGCAGC	GCTGCTCTTTCAGCATGAAAATTAGAACAGTAAACTT CCATCTG
pOPTN_E478G-EGFP	CTGATTTTCATGCTGG AAGAGCAGCGAGAG	CAGCATGAAAATCAGAACAGTAAACTTC
pEGFP-OPTN_{2 bp-AG}	CTGAGGACCCCACTGATAG GACTCCAGGCT	ATCAGTGGGGTCCTCAGATGACCTTTCT
pEGFP-OPTN_Q398X	GACACACAACAAGCTTCTTT AAGAACATAATA	AAGAAGCTTGTTGTGTGTCATCTGTAGC
pEGFP-OPTN_wt	AAA GAA TTC AAT GTC CCA TCA ACC TCT CAG	TTT TCT AGA TTA AAT GAT GCA ATC CAT CAC
pEGFP-OPTN_1-55	AAA GAA TTC AAT GTC CCA TCA ACC TCT CAG	TTT TCT AGA TCA GTG GGG TCC TTT CAG CTG GTG GTT CTC
pEGFP-OPTN_1-209	AAA GAA TTC AAT GTC CCA TCA ACC TCT CAG	TTT TCT AGA TTA CGT GCC AGT GGA GAC TGT TC
pEGFP-OPTN_1-424	AAA GAA TTC AAT GTC CCA TCA ACC TCT CAG	TTT TCT AGA TTA CTT CAG CAC TGC CCT GTC CA
pEGFP-OPTN_210-424	AAA GAA TTC AAT GGC ATT GTC TAA ATA TAG GAG	TTT TCT AGA TTA CTT CAG CAC TGC CCT GTC CA
pEGFP-OPTN_210-577	AAA GAA TTC AAT GGC ATT GTC TAA ATA TAG GAG	TTT TCT AGA TTA AAT GAT GCA ATC CAT CAC
pEGFP-OPTN_425-577	AAA GAA TTC AAT GGA ACT GAG TGA AAA ACT GGA	TTT TCT AGA TTA AAT GAT GCA ATC CAT CAC

Primer sequences used for constructing GFP-tagged optineurin mutant and deletion fragment expression plasmids are listed. The mutated nucleotides are labeled in boldface. The restriction enzyme sites that were used in the cloning are in italics.