Figure 6
From: Effects of mutations and deletions in the human optineurin gene
Apoptosis. RGC5 cells transfected with pEGFP-N1 (mock control) or one of optineurin expression vectors were incubated with caspase 3/7 substrate and Hoechst. A. Representative micrographs for GFP, and GFP-tagged optineurin wild type, E50K, R96L, L157A, E478G, 1–55, 1–148, 1–209, 1–398, 1–424, and 217–577 expressing cells are shown. The nuclei are stained in blue, the caspase3/7 positive cells (indicated by arrowheads) are in red and the transfected cells are in green (inset). Apoptosis was observed in cells expressing wild type, E50K, R96L, 2 bp-AG insertion (1–148) and Q398X (1–398) optineurin but not in those expressing L157A or remaining mutants and deletion fragments. Scale bar, 20 μm. B. Quantification of percentage of cells with apoptosis also showed similar trend as in A. *P < 0.0031.