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Figure 5 | SpringerPlus

Figure 5

From: Effects of mutations and deletions in the human optineurin gene

Figure 5

Transferrin uptake. RGC5 cells transfected with pEGFP-N1 (mock control) or one of the optineurin expression vectors were incubated with Texas red-transferrin (TR-Tf). Representative micrographs for GFP, and optineurin wild type (OPTNWT)-, OPTNE50K-, OPTNR96L-, OPTND474N-, OPTN1-148-, OPTN1-398-, OPTN210-424-, and OPTN217-577-GFP expressing cells are shown (A). The internalized TR-Tf is in red and the transfected cells are indicated as white stars (green channel is shown in the inset). Scale bar, 20 μm. The internalization of TR-Tf in transfected cells and non-transfected cells was quantified (B). Compared to GFP mock control and non-transfected normal control, cells expressing wild type, E50K, R96L, 2 bp-AG insertion (1–148), and Q398X (1–398) optineurin had a reduced TR-Tf uptake (*, P < 0.031). The uptake impairment phenotype was not observed with E478G mutation or any other deletion fragments. Scale bar, 20 μm.

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