Figure 3
From: Effects of mutations and deletions in the human optineurin gene
Foci formation in Neuro2A cells. Cells transfected with pEGFP-N1 (mock control), GFP-tagged optineurin wild type, mutants as well as deletion fragment constructs were observed by fluorescence microscopy 24 hours post transfection. Bright granular structures (foci, arrowheads) in perinuclear regions were prominently observed in cells expressing optineurin wild type, E50K, and R96L, but not in the cells transfected with other mutant or deletion constructs. Note that fragment 1–148 was expressed in the nucleus. Scale bar, 20 μm.