Figure 2

Foci formation in RGC5 cells. Cells transfected with pEGFP-N1 (mock control), GFP-tagged optineurin wild type, mutants as well as deletion fragment constructs were observed by fluorescence microscopy 24 hours post transfection. Bright granular structures (foci, arrowheads) in perinuclear regions were prominently observed in cells expressing optineurin wild type, E50K, and R96L, but not at all in those expressing D474N, E478G and deletion fragments 1–55, 1–148, 1–209, 1–398, and 1–424. In cells expressing mutant L157A and deletion fragments 217–577 and 425–577 (not shown), foci were typically not detected. Foci were noted occasionally, but they were small in size, low in number, and were not concentrated in the perinuclear area. Note nuclear location of deletion fragment 1–148. Scale bar, 20 μm.