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Table 1 Results of optimization

From: Validation of assay indicating method development of meloxicam in bulk and some of its tablet dosage forms by RP-HPLC

Column used Mobile phase Flow rate Wave length Observation Result
Microbondapak C18, 5 μm, 50 × 4.6 mm i.d. Methanol only 0.5 ml/min 254 nm Low resolution below 1. Method rejected
Phenomenex RP- C18, Luna 5 μm, 250 × 4.6 mm i.d. Acetonitrile only 0.8 ml/min 358 nm Resolution i.e. 1.2 but not satisfied Method rejected
Microbondapak C18, 5 μm, 50 × 4.6 mm i.d. Acetonitrile: phosphate buffer(pH7.8) = 70:30 1 ml/min 270 nm Poor resolution i.e. 1.5 Method rejected
Phenomenex RP- C18, Luna 5 μm, 250 × 4.6 mm i.d. Acetronitrile: phosphate buffer(pH 5.0 = 60:40 1 ml/min 270 nm Poor resolution i.e. 1.2 Method rejected
Develosil ODS HG-5 RP C18, 5 μm, 15cm × 4.6 mm i.d. Acetonitrile: phosphate buffer(pH 3.4) (60:40) 1 ml/min 268 nm Sharp peak and good resolution i.e. 2.3 Method accepted
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