Table 1 Number of primer sets and information of primer sets for PCR and sequencing
Number of primer sets | Primer name | Sequences (5′-> 3′) | PCR or Sequencing | Origin |
|---|---|---|---|---|
1 | sGluN-F1 | ATC ACC GGC ATC AAC GAC CC | PCR and Sequencing | This study |
sGluN-R1 | GCT GCA AAA GCC AGC TGC AT | PCR and Sequencing | This study | |
2 | sGluN-F2 | ACT GCT TCA AGT CGG CAT TT | PCR and Sequencing | This study |
sGluN-R2 | GGG TCG TTG ATG CCG GTG AT | PCR and Sequencing | This study | |
3 | sGluN-F3 | GAG CAG GTG TTC AAG GAT GC | PCR and Sequencing | This study |
sGluN-R3 | AAA TGC CGA CTT GAA GCA GT | PCR and Sequencing | This study | |
4 | Uni-primer | TCACAGAAGTATGCCAAGCGA | PCR and Sequencing | *DNA Walking SpeedUp™ Premix Kit |
Chum_NMDAR_5o | TGATGTGGGCCGACTCGTTCC | PCR and Sequencing | This study | |
Chum_NMDAR_5i | AGCTCTTTGGCCTCTAGAAGCAGC | Sequencing | This study | |
5 | Uni-primer | TCACAGAAGTATGCCAAGCGA | PCR and Sequencing | *DNA Walking SpeedUp™ Premix Kit |
Chum_NMDAR_3o | CTGGCTGCCTTCCTGGTGC | PCR and Sequencing | This study | |
Chum_NMDAR_3i | TTAGCACCATGTACCGCCACATGG | Sequencing | This study |